Coding
AtPAL2

Part:BBa_K2783010:Design

Designed by: Matthijs Jonathan Tadema   Group: iGEM18_Groningen   (2018-10-07)


Phenylalanine ammonia lyase 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 337
    Illegal AgeI site found at 652
    Illegal AgeI site found at 676
    Illegal AgeI site found at 1651
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 184
    Illegal BsaI.rc site found at 253
    Illegal BsaI.rc site found at 439
    Illegal SapI.rc site found at 1873
    Illegal SapI.rc site found at 1999


Design Notes

DNA is codon optimized for E.coli.


Source

Synthesized, protein sequence based on genomic sequence of Arabidopsis thaliana.

References